The effect of bone graft substitute in healing fractures with bone defects through examination of alkaline phosphatase and radiology in the murine model (Rattus norvegicus) Wistar strain

Background: A significant bone defect is a condition wherein the cannot repair spontaneously. Therefore, replacing defects with substitution remains reconstructive concern for orthopaedic surgeons. Bone Graft Substitution (BGS) are classified broadly, such as grafts (autograft, allograft, and xenograft) synthetic ceramics (hydroxyapatite, calcium sulphate). This study aims to determine effect of various Substitute on healing process assessed based area callus formation levels alkaline phosphatase (ALP). Methods: The design was an in vivo laboratory experimental approach randomized post-test only control group design. 36 animals that matched inclusion criteria were divided into five groups, each one positive group, negative three treatment group. graft used this ceramic, namely Synthetic HA-Ca10(PO4)6(OH)2 - BONGROS®, Nanocrystalline HA-CaSO4-PEROSSAL®, also hydroxyapatite Bovine. After selecting rats, we performed osteotomy femur made defect. 30 days, murine models harvested. Then, measure using radiological examination ALP level serum Results: From Callus formation, HA-CaSO4 highest (86.54 ± 4.24604) compared other groups significantly (p:0.021) increase than groups. from level, Bovine (9.287 0.58586) but did not compare K-neg, second higher KP-1, it has rather K-Neg. Conclusion: Substituted good material can fracture model rats defects.